Here we provide a curated list of antibodies compatible with iDISCO.
If you have had success with other antibodies and would like to contribute to the list
please email us at firstname.lastname@example.org (credit will be given).
All antibodies listed are compatible with the methanol protocol.
APP- abcam ab32136
1:250 for E14 embryos.
Aquaporin2- Santa Cruz sc-9882
1:20 for adult mouse kidney.
Arc- Synaptic Systems 156003
1:500 to 1:1000 for adult mouse brain.
beta-Catenin- Abcam ab6302
1:50 for adult mouse kidney.
beta-Galatosidase- Abcam ab9361
1:200 for E14.5 mouse embryos.
cFos- Santa Cruz sc-52
1:200 for adult mouse brain and spinal cord.
Cleaved Caspase 3- Cell Signaling 9661
1:500 for E10.5 to E16.5 mouse embryos and adult mouse brain.
Cux1 (CDP)- Santa Cruz sc-13024 (contributed by Sean Hsiang-Chih, Huda Zoghbi Lab)
1:200 adult mouse brain
E-Cadherin- Cell Signaling 3195
1:20 for adult mouse stomach and kidney.
Egr-1- Santa Cruz sc-110
1:200 in mouse adult brain
FoxP2- Abcam ab16046
1:500 for adult mouse brains and E13 mouse embryos.
GFP- Aves GFP-1020
E14 embryos, adult mouse brains. Dilution varies by GFP expression level. 1:2000 for sparse AAV-GFP injections.
Note: we recommend this antibody but Abcam ab290 and Invitrogen A11122 also provide decent results. The Abcam rabbit anti-GFP can be used at higher concentration (up to 1:200) if the coverage of the Aves antibody is not sufficient.
Iba1- Wako 019-19741
Adult mouse brain, 1:200.
Laminin- Sigma L9393
1:50 for adult mouse muscle and kidney
Mash1- BD Biosciences 556604 (contributed by Eduardo Arteaga)
1:100 on mouse E12 embryos
N-Cadherin- Abcam ab12221
1:50 for adult mouse heart.
Nephrin- R&D AF3159
1:400 for adult mouse kidney
Covance PRB-575C (now Biolegend 841001)
1:200 for E12.5 to E16.5 mouse embryos
Parvalbumin- Swant PV 25 (now PV27)
1:1000 for E12.5 and E14.5 embryos.
1:100 for adult mouse brains (only small blocs, about 2 mm on each side, limited diffusion otherwise).
Phospho-cJun- Cell Signaling 3270 and 9261
1:250 Adult mouse DRG and spinal cord.
Phospho-Histone-H3- Abcam ab5176
1:200 E14.5 mouse embryos and adult mouse spleen.
Phospho-S6 (Ser235/236)- Cell Signaling 4858
1:200 adult mouse brain.
Phospho-S6 (Ser244/247)- Life Technologies 44923G
1:300 for adult mouse brain.
RFP (tdtomato/mCherry)- Rockland 600-401-379
1:1000 to 1:200. E12.5 mouse embryos, adult mouse brain. Dilution varies by RFP expression level.
Ret- R&D AF482
1:400 on E12.5 mouse embryos
Robo3- R&D AF3076
1:500 for E11.5 and E12.5 embryos.
Sox2- Cell Signaling 2748
1:500 adult mouse brain
1:1000 mouse E12 embryos (contributed by Eduardo Arteaga)
Tbr2- Cell Signaling 3728
1:1000 on mouse E12 embryos. (contributed by Eduardo Arteaga)
TrkA- R&D AF1056
1:400 for E12.5 to E16.5 embryos.
Note: this antibody has a short half-life at 4°c. Keep at -80°c and unfreeze aliquots for immediate use.
TrkB- R&D AF1494
1:400 for E12.5 mouse embryos. TrkB doesn’t give nice axonal staining, because of the expression in other structures.
TrkC- R&D AF1404
1:400 for E12.5 and E14.5 mouse embryos.
Tuj1- Covance MRB-435P
1:200 for mouse E12.5 to E16.5 embryos. Not usable on adult tissues.
Tyrosine Hydroxylase- Millipore AB152
1:1000 to 1:100 E15.5, E18 mouse embryos, P0 mouse brain, adult mouse brain half, adult mouse intestine, adult mouse skin.